Novel GUS expression patterns following transposition of an enhancer trap Ds element in Arabidopsis.
Mol Gen Genet
; 249(4): 357-65, 1995 Dec 10.
Article
en En
| MEDLINE
| ID: mdl-8552040
Enhancer trap derivatives of the maize Dissociation (Ds) transposon were introduced into Arabidopsis thaliana. The enhancer trap Ds was so designed that upon transposition to sites containing regulatory sequences in adjacent genomic DNA, transcription of a Ds-borne beta-glucuronidase (GUS) gene would be activated. Sixty percent of all transposition events were associated with GUS expression patterns including one linked to a mutant phenotype. Patterns of GUS expression were found in various organs and were stably inheritable in the F4 and F5 progenies. These results demonstrate the potential value of the technique as a means for detection of developmentally regulated genes and analysis of their function. The enhancer trap construct used in our experiments, as well as the seeds of primary transformants are publicly available.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Elementos Transponibles de ADN
/
Elementos de Facilitación Genéticos
/
Arabidopsis
/
Glucuronidasa
Idioma:
En
Revista:
Mol Gen Genet
Año:
1995
Tipo del documento:
Article
País de afiliación:
Reino Unido
Pais de publicación:
Alemania