Transneuronal spread of the pseudorabies virus after injection into the central nucleus of the amygdala in the rat.
Gene Ther
; 1 Suppl 1: S74, 1994.
Article
en En
| MEDLINE
| ID: mdl-8542420
The pseudorabies virus (PRV) is a swine alpha herpes virus that is widely used as a neural tracer because of its marked neurotropism and transneuronal transmissibility (Card et al., 1991, 1992; Strack and Loewy 1990). PRV has been used to retrogradely identify spinal cord and brainstem connections to various peripheral organs, but few anatomical studies have used CNS inoculation of PRV to investigate intrinsic brain connectivity. Improved knowledge of the mode and temporal pattern of transneuronal spread is essential for interpretation of PRV tracing studies, and is also a prerequisite to the use of this and other herpes viruses as vectors in the CNS. This study investigated the distribution of PRV labelling in the CNS at various time points after its injection into the central nucleus of the amygdala (CA). The results indicate that detection of PRV in a retrogradely labelled site at any given time after injection is not only a function of the number of synapses in the pathway from the injection site, but is also highly dependent on the axon lengths involved, much more than would be expected if fast axonal transport were the limiting factor. In addition, the window of time during which PRV may be detected in a given site is limited ultimately by neuronal destruction.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Herpesvirus Suido 1
/
Amígdala del Cerebelo
/
Neuronas
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Gene Ther
Asunto de la revista:
GENETICA MEDICA
/
TERAPEUTICA
Año:
1994
Tipo del documento:
Article
País de afiliación:
Estados Unidos
Pais de publicación:
Reino Unido