Rhizobium tropici chromosomal citrate synthase gene.
Appl Environ Microbiol
; 61(11): 3992-7, 1995 Nov.
Article
en En
| MEDLINE
| ID: mdl-8526514
Two genes encoding citrate synthase, a key enzyme in the Krebs cycle, have been found in Rhizobium tropici. One of them is in the bacterial chromosome, while the other is in the symbiotic plasmid. We sequenced the chromosomal gene and found that it is very similar to the previously reported plasmidic gene sequence in its structural region but not in its regulatory region. The chromosomal gene is able to complement an Escherichia coli citrate synthase mutant. In R. tropici, a mutant in the chromosomal citrate synthase gene has a diminished citrate synthase activity (in free-living bacteria), a diminished nodulation capacity, and forms nitrogen-fixing nodules. In contrast, the citrate synthase double mutant forms ineffective nodules devoid of bacteroids and forms less nodules than the single chromosomal mutant. It is inferred that both genes are functional and required during the nodulation process in R. tropici.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Rhizobium
/
Cromosomas Bacterianos
/
Citrato (si)-Sintasa
/
Genes Bacterianos
Idioma:
En
Revista:
Appl Environ Microbiol
Año:
1995
Tipo del documento:
Article
Pais de publicación:
Estados Unidos