Relaxation of supercoiled DNA by aminomethyl trimethylpsoralen and UV photons: action spectrum.
Photochem Photobiol
; 57(4): 648-54, 1993 Apr.
Article
en En
| MEDLINE
| ID: mdl-8506393
An action spectrum for the relaxation of supercoiled plasmid DNA (induction of the first single-strand break) by photoactivated 4'-aminomethyl-4,5',8-trimethylpsoralen (AMT) has been determined using monochromatic UV photons from 254 to 405 nm. The spectrum of AMT-induced plasmid DNA relaxation fits closely with the absorbance spectrum of AMT in the spectral region between 313 nm and 405 nm but deviates at wavelengths shorter than 313 nm. This assay also reveals that the psoralen photosensitization reaction with DNA also produces piperidine-labile sites. Addition of mannitol and azide partially quenches the supercoil relaxation reaction, evidence for a role of Type II photosensitization pathway.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Trioxsaleno
/
Rayos Ultravioleta
/
ADN Superhelicoidal
Idioma:
En
Revista:
Photochem Photobiol
Año:
1993
Tipo del documento:
Article
Pais de publicación:
Estados Unidos