Nucleotide sequence of a genomic gene encoding tritin, a ribosome-inactivating protein from Triticum aestivum.
Plant Mol Biol
; 22(1): 171-6, 1993 Apr.
Article
en En
| MEDLINE
| ID: mdl-8499616
A genomic gene of tritin, a ribosome-inactivating protein (RIP) from Triticum aestivum, was cloned using a barley RIP gene as a probe. The 5'-non-coding region has potential TATA boxes and three sequences homologous to the binding sequence of the transcriptional activator protein Opaque-2 which activates maize RIP gene expression. The cloned DNA encoded tritin consists of 275 amino acids with no secretion signal sequence. The coding region of tritin was expressed in Escherichia coli using lac promoter and yielded a protein similar to the native one, as determined by SDS-polyacrylamide gel electrophoresis and immunological analysis.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas de Plantas
/
Triticum
/
Inhibidores de la Síntesis de la Proteína
/
Genes de Plantas
Idioma:
En
Revista:
Plant Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
/
BOTANICA
Año:
1993
Tipo del documento:
Article
Pais de publicación:
Países Bajos