Pertussis toxin expression in Drosophila alters the visual response and blocks eating behaviour.
Cell Signal
; 5(2): 187-207, 1993 Mar.
Article
en En
| MEDLINE
| ID: mdl-8499226
Pertussis toxin inactivates certain G-proteins by introducing an ADP-ribose group near the carboxyl-terminus of the alpha-subunit. The major pertussis toxin substrate in Drosophila tissues is Go alpha. We introduced a pertussis toxin gene under control of the hsp70 heat-shock promoter into the Drosophila genome. When heat-shocked, transformed flies produce active pertussis toxin which ADP-ribosylates endogenous Go alpha. Pertussis toxin is expressed in photoreceptors, in the lamina of the eye and in epithelial cells lining the gut. As expected from the absence of Go alpha in photoreceptors, pertussis toxin does not affect the photoreceptor component of the Drosophila visual response. However, it abolishes light on- and off-transients in the electroretinogram. These transients normally arise from the lamina, a tissue where Go alpha transcripts have been detected. Pertussis toxin expression also blocks embryonic development and shortens the lifetime of adult Drosophila. Following heat-shock, transformed adults are active, but they fail to take up nutrients because they stop eating. High energy metabolites are significantly depleted shortly after pertussis toxin expression is induced and the flies die within 48 h.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Visión Ocular
/
Factores de Virulencia de Bordetella
/
Toxina del Pertussis
/
Drosophila melanogaster
/
Conducta Alimentaria
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Cell Signal
Año:
1993
Tipo del documento:
Article
Pais de publicación:
Reino Unido