[Action of cycloheximide on RNA synthesis and the formation of structures in the Venezuelan equine encephalomelitis virus]. / Deistvie tsiklogeksimida na sintez RNK i formirovanie struktur virusa venesuel'skogo éntsefalomielita loshadei
Antibiotiki
; 22(3): 252-8, 1977 Mar.
Article
en Ru
| MEDLINE
| ID: mdl-848919
The studies showed that the effect of cycloheximide on reproduction of the virus of Venezuelan equine encephalitis (VEE) depended on the time of the antibiotic administration. When the antibiotic was added 3--5 hours after the inoculation (after formation of the virus RNA-polymerase), accumulation of virus RNA and 60--100 S virus structures was observed in the cells, while virus nucleotides and infection virus did not practically form. The 60--100 S structures included the cell proteins. They were resistant to EDTA and their swimming density in cesium chloride was 1.3--1.4 gm/cm3, it was characteristic of RNA of a non-ribosomic nature. According to these properties 60--100 S structures may be classified as belonging to the "hybrid" RNA analogous to the RNA complexes of VEE virus with the cell proteins. Probably addition of cycloheximide provided conditions in the infected cells for increase of the "hybrid", structural pool since the synthesis of the structural virus proteins was suppressed.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
ARN Viral
/
Cicloheximida
/
Virus de la Encefalitis Equina Venezolana
País/Región como asunto:
America do sul
/
Venezuela
Idioma:
Ru
Revista:
Antibiotiki
Año:
1977
Tipo del documento:
Article
Pais de publicación:
Rusia