Strand breaks in DNA induced by a thiol/Fe(III)/O2 mixed-function oxidase system and its protection by a yeast antioxidant protein.

Kwon, S J; Kim, K; Kim, I H; Yoon, I K; Park, J W

Kwon, S J; Kim, K; Kim, I H; Yoon, I K; Park, J W.

Afiliación

Kwon SJ; Department of Biochemistry, College of Natural Sciences, Kyungpook National University, Taegu, Korea.

Biochem Biophys Res Commun ; 192(2): 772-7, 1993 Apr 30.

Article en En | MEDLINE | ID: mdl-8484783

RESUMEN

Strand breaks can be produced in pBluescript plasmid DNA and calf thymus DNA by a mixed-function oxidase (MFO) system comprised of Fe3+, O2, and dithiothreitol as an electron donor. Superoxide dismutase does not block this damage whereas a 27-KDa yeast antioxidant protein specifically inhibits strand breaks in DNA induced by the dithiothreitol MFO system. In contrast, this protein does not inhibit strand breaks in DNA induced by an ascorbate MFO system although catalase inhibits damage in DNA caused by both MFO systems. Based on the specificity of this protein, we propose that the antioxidant protein functions as a sulfur radical scavenger.

Asunto(s)

Antioxidantes/farmacología; Daño del ADN; Proteínas Fúngicas/farmacología; Oxigenasas de Función Mixta/farmacología; Proteínas de Neoplasias; Peroxidasas; Animales; Bovinos; Daño del ADN/efectos de los fármacos; Ditiotreitol/farmacología; Hierro/farmacología; Oxigenasas de Función Mixta/antagonistas & inhibidores; Oxígeno/farmacología; Peroxirredoxinas

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Peroxidasas / Daño del ADN / Proteínas Fúngicas / Oxigenasas de Función Mixta / Proteínas de Neoplasias / Antioxidantes Límite: Animals Idioma: En Revista: Biochem Biophys Res Commun Año: 1993 Tipo del documento: Article Pais de publicación: Estados Unidos

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