A sequential staining technique for karyotypic analysis of interspecific somatic cell hybrids.

Keherly, M J; Papaconstantinou, J; McCombs, J L

Keherly, M J; Papaconstantinou, J; McCombs, J L.

Afiliación

Keherly MJ; Department of Human Biological Chemistry and Genetics, University of Texas, Medical Branch, Galveston 77555-0359.

Exp Cell Res ; 205(2): 361-4, 1993 Apr.

Article en En | MEDLINE | ID: mdl-8482340

RESUMEN

We describe a sequential staining technique for the karyotypic analysis of interspecific mouse x human somatic cell hybrids. Fluorescence in situ hybridization of samples, previously stained using standard trypsin/Giemsa protocols, was instrumental in the identification of human chromosomes present in hybrid lines. This procedure not only provided a simple distinction between human and mouse chromosomes, but it also allowed the visualization and monitoring of human sequences present in interspecific translocations and subchromosomal fragments.

Asunto(s)

Bandeo Cromosómico/métodos; Células Híbridas/ultraestructura; Hibridación in Situ/métodos; Cariotipificación/métodos; Animales; Colorantes Azulados; Fijadores; Humanos; Ratones

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bandeo Cromosómico / Hibridación in Situ / Células Híbridas / Cariotipificación Límite: Animals / Humans Idioma: En Revista: Exp Cell Res Año: 1993 Tipo del documento: Article Pais de publicación: Estados Unidos

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