Regional differences in lectin binding patterns of vestibular hair cells.
Hear Res
; 65(1-2): 151-63, 1993 Feb.
Article
en En
| MEDLINE
| ID: mdl-8458748
Surface glycoconjugates of hair cells and supporting cells in the vestibular endorgans of the bullfrog were identified using biotinylated lectins with different carbohydrate specificities. Lectin binding in hair cells was consistent with the presence of glucose and mannose (CON A), galactose (RCA-I), N-acetylglucosamine (WGA), N-acetylgalactosamine (VVA), but not fucose (UEA-I) residues. Hair cells in the bullfrog sacculus, unlike those in the utriculus and semicircular canals, did not strain for N-acetylglucosamine (WGA) or N-acetylgalactosamine (VVA). By contrast, WGA and, to a lesser extent, VVA, differentially stained utricular and semicircular canal hair cells, labeling hair cells located in peripheral, but not central, regions. In mammals, WGA uniformly labeled Type I hair cells while labeling, as in the bullfrog, Type II hair cells only in peripheral regions. These regional variations were retained after enzymatic digestion. We conclude that vestibular hair cells differ in their surface glycoconjugates and that differences in lectin binding patterns can be used to identify hair cell types and to infer the epithelial origin of isolated vestibular hair cells.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Glicoconjugados
/
Vestíbulo del Laberinto
/
Células Ciliadas Auditivas
/
Lectinas
Límite:
Animals
Idioma:
En
Revista:
Hear Res
Año:
1993
Tipo del documento:
Article
Pais de publicación:
Países Bajos