Resolution and quantification of four metallothionein isoforms from rabbit kidney cells.
Protein Expr Purif
; 4(1): 38-44, 1993 Feb.
Article
en En
| MEDLINE
| ID: mdl-8425107
Reversed-phase high-performance liquid chromatography coupled with an on-line integrating chromatographic data system was used to separate and quantify the four major isometallothioneins MT-1a, MT-2a, MT-2d, and MT-2e present in metallothionein samples from cultured rabbit kidney cells as prepared by gel-filtration and/or ion-exchange chromatography. The standard curves for each of these isoforms showed closely comparable linear correlations between the amount of protein applied to the column and their integrated absorbance peak area at 220 nm. The lower limit of quantification is 30 pmol, sufficient to assess basal isometallothionein concentrations and to follow their variation upon metal exposure.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Riñón
/
Metalotioneína
Tipo de estudio:
Diagnostic_studies
/
Evaluation_studies
Límite:
Animals
Idioma:
En
Revista:
Protein Expr Purif
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
1993
Tipo del documento:
Article
País de afiliación:
Suiza
Pais de publicación:
Estados Unidos