Immobilisation of levansucrase on calcium phosphate gel strongly increases its polymerase activity.
Carbohydr Res
; 244(1): 129-36, 1993 May 21.
Article
en En
| MEDLINE
| ID: mdl-8393383
The catalytic properties of levansucrase bound to hydroxyapatite were studied as a possible model for enzyme behaviour when associated in vivo to matrices such as the cell wall of bacteria or tooth surfaces. The activity of the immobilised enzyme was mainly directed towards its polymerase activity. The yield of levan reached 85%. The kcat of the enzyme for sucrose transformation was increased and the Km for this substrate was unmodified. These properties allow the design of a system for the large-scale production of high-molecular-weight branched-chain levan in vitro in high yield.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Enzimas Inmovilizadas
/
Fructanos
/
Hexosiltransferasas
Idioma:
En
Revista:
Carbohydr Res
Año:
1993
Tipo del documento:
Article
País de afiliación:
Francia
Pais de publicación:
Países Bajos