Characterization of kinetoplast DNA from Phytomonas serpens.
J Eukaryot Microbiol
; 40(3): 317-22, 1993.
Article
en En
| MEDLINE
| ID: mdl-8389640
The restriction enzyme digestion of kinetoplast DNA from four Phytomonas serpens isolates shows an overall similar band pattern. One minicircle from isolate 30T was cloned and sequenced, showing low levels of homology but the same general features and organization as described for minicircles of other trypanosomatids. Extensive regions of the minicircle are composed by G and T on the H strand. These regions are very repetitive and similar to regions in a minicircle of Crithidia oncopelti and to telomeric sequences of Saccharomyces cerevisiae. Conserved Sequence Block 3, present in all trypanosomatids, is one nucleotide different from the consensus in P. serpens and provides a basis to differentiate P. serpens from other trypanosomatids. Electron microscopy of kinetoplast DNA evidenced a network with organization similar to other trypanosomatids and the measurement of minicircles confirmed the size of about 1.45 kb of the sequenced minicircle.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
ADN Circular
/
ADN Protozoario
/
Trypanosomatina
Límite:
Animals
Idioma:
En
Revista:
J Eukaryot Microbiol
Asunto de la revista:
MICROBIOLOGIA
/
PARASITOLOGIA
Año:
1993
Tipo del documento:
Article
País de afiliación:
Brasil
Pais de publicación:
Estados Unidos