Characterization of developmentally-regulated nucleases in promastigotes and amastigotes of Leishmania mexicana.
FEMS Microbiol Lett
; 107(1): 53-8, 1993 Feb 15.
Article
en En
| MEDLINE
| ID: mdl-8385643
The parasitic protozoon Leishmania mexicana was examined for the presence of 3'-nucleotidase/nuclease using substrate SDS-PAGE. Two activities were detected: one with an apparent molecular mass of 40 kDa, and a doublet of 29/31 kDa. The two enzymes showed differences in their levels of expression in the two life-cycle stages of parasite examined: the 29/31 kDa doublet was detected at 60-fold higher levels in the pathogenic amastigote stage, but was also expressed by promastigotes, whereas the 40 kDa activity was only detected in promastigotes. Both were capable of hydrolysing a variety of substrates including 3'-AMP and poly(A). However, the 29/31 kDa form showed a broader, unique substrate specificity in that it was also capable of digesting double-stranded RNA and DNA.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Leishmania mexicana
/
Proteínas Protozoarias
/
Nucleotidasas
Límite:
Animals
País/Región como asunto:
Mexico
Idioma:
En
Revista:
FEMS Microbiol Lett
Año:
1993
Tipo del documento:
Article
Pais de publicación:
Reino Unido