Characterization of two cytosolic diacylglycerol kinase forms.
J Neurochem
; 60(4): 1212-9, 1993 Apr.
Article
en En
| MEDLINE
| ID: mdl-8384245
Two forms of rat brain cytosolic diacylglycerol kinase (EC2.7.1.107) were separated by heparin-agarose column chromatography. These forms, designated DGK-I and DGK-II, were not interconvertible as determined by rechromatography. DGK-I and DGK-II had respective molecular masses of 88 and 180 kDa, as measured by Sepharose 6B chromatography. Both forms preferred diacylglycerol over monoacylglycerol and were insensitive to R59022. DGK-II, but not DGK-I, was activated by an activator substance prepared from chicken egg yolk. DGK-II was activated by a rat brain cytosolic activator and was exclusively sensitive to 5'-AMP-mediated inactivation. Further studies revealed that these two forms had the following distinct characteristics: (a) substrate specificity, (b) inhibition by heparin, (c) sensitivity to lysine-containing polyamino acids, and (d) responses to different phospholipids. In general, DGK-II was more responsive to various inhibitors and activators, making it a prime candidate for a regulatable enzyme.
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01-internacional
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MEDLINE
Asunto principal:
Fosfotransferasas
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Animals
Idioma:
En
Revista:
J Neurochem
Año:
1993
Tipo del documento:
Article
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Reino Unido