Typing of human papillomavirus DNAs by restriction endonuclease mapping of the PCR products.
J Virol Methods
; 41(1): 29-36, 1993 Jan.
Article
en En
| MEDLINE
| ID: mdl-8381794
The polymerase chain reaction (PCR) for the diagnosis of human papillomavirus (HPV) infections, and in particular for the study of cervical HPV-associated lesions, is used widely. We identified a novel set of universal primers that are able to amplify a fragment spanning the E1 open reading frame (ORF) from different mucosotropic HPV types. A restriction endonuclease digestion of the amplified products is suggested for accurate typing. In particular, AluI digestion of the amplified fragments yields a distinctive fragment pattern for each 'high-risk' (16, 18, 31 and 33) HPV sequence, thus distinguishing them from 'low-risk' (6b and 11) HPV sequences.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Papillomaviridae
/
Virología
/
ADN Viral
/
Reacción en Cadena de la Polimerasa
Tipo de estudio:
Diagnostic_studies
/
Etiology_studies
/
Prognostic_studies
Límite:
Female
/
Humans
Idioma:
En
Revista:
J Virol Methods
Año:
1993
Tipo del documento:
Article
País de afiliación:
Italia
Pais de publicación:
Países Bajos