A monoclonal antibody recognizing a differentiation marker on rat gonocytes.
J Reprod Immunol
; 23(1): 93-108, 1993 Jan.
Article
en En
| MEDLINE
| ID: mdl-8381490
Monoclonal antibodies (MAb) were raised against a testicular membrane fraction from 18-day post coitum (p.c.) rat testes. One antibody, designated 4B6.3E10 (mu, kappa), was obtained which specifically reacted with gonocytes in the fetal testis. No significant cross-reactivity with other tissues from the 18-day p.c. embryo was found. MAb 4B6.3E10 was reactive with rat gonocytes from 17-day p.c. until the day of birth. Germ cells at later stages of testis development did not show any labelling. The epitope recognized by 4B6.3E10 is a carbohydrate as periodate treatment leads to a loss of reactivity of the antibody. By SDS-PAGE and Western blotting of proteins extracted from a testicular membrane fraction from 18-day p.c. testes, MAb 4B6.3E10 was found to recognize at least 3 protein moieties with apparent molecular weights in the ranges of 80-100, 120, 160-180 kDa (either under reducing- or non-reducing conditions). The results suggest that MAb 4B6.3E10 recognizes a specific differentiation marker for fetal rat gonocytes.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Testículo
/
Antígenos de Diferenciación
/
Ratones Endogámicos BALB C
Límite:
Animals
Idioma:
En
Revista:
J Reprod Immunol
Año:
1993
Tipo del documento:
Article
País de afiliación:
Países Bajos
Pais de publicación:
Irlanda