Fosfomycin determination in serum by capillary zone electrophoresis with indirect ultraviolet detection.
J Chromatogr
; 616(2): 311-6, 1993 Jul 02.
Article
en En
| MEDLINE
| ID: mdl-8376512
Capillary zone electrophoresis with indirect ultraviolet detection was used for the determination of fosfomycin in serum. Running buffer consisted of a mixture of 200 mM sodium borate with 10 mM phenylphosphonic acid used as ultraviolet absorbing background electrolyte. Relationships between the pH of the buffer and the efficiency of the separation (migration times and selectivities) or the sensitivity of detection were investigated. The method was then validated over a 10-100 micrograms ml-1 concentration range to be applied to further therapeutic drug monitoring. The choice of ethylphosphonic acid as internal standard is discussed. The specificity and the linearity of the technique are demonstrated. The inter-day precision was satisfactory with a relative standard deviation of less than 2%. Accuracy was calculated with a standard error near 0.5 and 18% for 100 and 10 micrograms ml-1, respectively.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Fosfomicina
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Revista:
J Chromatogr
Año:
1993
Tipo del documento:
Article
País de afiliación:
Francia
Pais de publicación:
Países Bajos