HIV DNA and antibodies in syringes from injecting drug users: a comparison of detection techniques.
AIDS
; 7(7): 925-31, 1993 Jul.
Article
en En
| MEDLINE
| ID: mdl-8357554
OBJECTIVE: Direct HIV testing of individual injecting drug users is not always feasible. As an alternative, we have evaluated the sensitivity and specificity of several techniques for detecting HIV-1-specific products in used syringes. DESIGN: Polymerase chain reaction (PCR) and antibody-capture assays were compared using syringes prepared with blood from HIV-1-positive and -negative individuals. METHODS: PCR sensitivity was maximized, enabling detection of single copies of HIV-1-specific proviral DNA. The limits of detection from used syringes were determined for PCR by diluting extracts and correlated to CD4+ cell counts. Similarly, limits of detection were determined for enzyme immunoassays (EIA) and Western blot. RESULTS: All techniques were highly specific, although with PCR false-positives were detected occasionally. EIA proved more sensitive than Western blot in detecting needles containing HIV-1-infected individuals' blood. Even after prolonged storage of syringes at room temperature, EIA was equal to or better than PCR as an HIV-1 detection technique. The most sensitive method for detecting HIV-1 was the viral-based EIA when the recommended predilution step was omitted. CONCLUSIONS: EIA proved preferable to PCR because of their higher sensitivity, absence of false-positives and easier sample preparation and analysis.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Jeringas
/
ADN Viral
/
Anticuerpos Anti-VIH
/
Abuso de Sustancias por Vía Intravenosa
/
Seropositividad para VIH
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Revista:
AIDS
Asunto de la revista:
SINDROME DA IMUNODEFICIENCIA ADQUIRIDA (AIDS)
Año:
1993
Tipo del documento:
Article
Pais de publicación:
Reino Unido