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Ixr1, a yeast protein that binds to platinated DNA and confers sensitivity to cisplatin.
Brown, S J; Kellett, P J; Lippard, S J.
Afiliación
  • Brown SJ; Department of Chemistry, Massachusetts Institute of Technology, Cambridge 02139.
Science ; 261(5121): 603-5, 1993 Jul 30.
Article en En | MEDLINE | ID: mdl-8342024
Structure-specific recognition proteins (SSRPs) bind to DNA containing intrastrand cross-links formed by the anticancer drug cisplatin. A yeast gene encoding an SSRP, designated IXR1, was cloned and sequenced. The Ixr1 protein, a member of the high mobility group-box protein family, bound specifically to DNA modified with cisplatin but not inactive platinum compounds. A yeast strain with an inactivated IXR1 gene was half as sensitive to cisplatin and accumulated one-third as many platinum-DNA lesions after treatment with cisplatin as the parental strain. These findings suggest that SSRPs play a role in mediating the cytotoxicity of cisplatin.
Asunto(s)
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Saccharomyces cerevisiae / ADN / ADN de Hongos / Proteínas Fúngicas / Proteínas del Grupo de Alta Movilidad / Cisplatino / Aductos de ADN / Proteínas de Saccharomyces cerevisiae / Proteínas de Unión al ADN Tipo de estudio: Diagnostic_studies Idioma: En Revista: Science Año: 1993 Tipo del documento: Article Pais de publicación: Estados Unidos
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Saccharomyces cerevisiae / ADN / ADN de Hongos / Proteínas Fúngicas / Proteínas del Grupo de Alta Movilidad / Cisplatino / Aductos de ADN / Proteínas de Saccharomyces cerevisiae / Proteínas de Unión al ADN Tipo de estudio: Diagnostic_studies Idioma: En Revista: Science Año: 1993 Tipo del documento: Article Pais de publicación: Estados Unidos