Isolation and purification of amyloglucosidase from Halobacterium sodomense.

Chaga, G; Porath, J; Illéni, T

Chaga, G; Porath, J; Illéni, T.

Afiliación

Chaga G; Biochemical Separation Centre, Uppsala University, Sweden.

Biomed Chromatogr ; 7(5): 256-61, 1993.

Article en En | MEDLINE | ID: mdl-8305855

RESUMEN

Amyloglucosidase from Halobacterium sodomense was purified by a combination of hydrophobic interaction chromatography and immobilized metal ion affinity chromatography at analytical and preparative scale with 75% recovery. The enzyme was found to be a dimer of two different subunits with molecular weights of 72,000 and 82,000 D, respectively, combining in a 175,000 D native protein. The specific activity, KM, and amino acid composition of the enzyme was determined.

Asunto(s)

Glucano 1,4-alfa-Glucosidasa/aislamiento & purificación; Halobacterium/enzimología; Cromatografía de Afinidad; Cromatografía en Gel; Cromatografía por Intercambio Iónico; Glucano 1,4-alfa-Glucosidasa/metabolismo; Metales; Peso Molecular

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Glucano 1,4-alfa-Glucosidasa / Halobacterium Idioma: En Revista: Biomed Chromatogr Año: 1993 Tipo del documento: Article País de afiliación: Suecia Pais de publicación: Reino Unido

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