Controlled high-level expression of the lon gene of Escherichia coli allows overproduction of Lon protease.
Gene
; 136(1-2): 237-42, 1993 Dec 22.
Article
en En
| MEDLINE
| ID: mdl-8294008
Lon protease from Escherichia coli is an ATP-dependent protease which plays important roles in regulating the levels of specific proteins and in eliminating abnormal proteins. A major problem of working with Lon protease, the inability to substantially overproduce the enzyme, has been overcome by placing the lon gene under the control of an inducible trp promoter within a copy-number-controllable plasmid. Induction resulted in higher levels of production of the protease (approximately 100 micrograms/ml of cell culture) than were previously possible. The enzyme has been purified to apparent homogeneity and shown to possess the characteristic ATP-dependent proteolytic activity. Sequence verification during DNA manipulations revealed differences from two previously published sequences for the lon gene.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Serina Endopeptidasas
/
Proteínas de Escherichia coli
/
Proteasa La
/
Escherichia coli
/
Proteínas de Choque Térmico
Idioma:
En
Revista:
Gene
Año:
1993
Tipo del documento:
Article
Pais de publicación:
Países Bajos