Intrinsic procoagulant surface induced by hypercholesterolaemia on rabbit aortic endothelium.
Blood Coagul Fibrinolysis
; 4(5): 743-52, 1993 Oct.
Article
en En
| MEDLINE
| ID: mdl-8292724
The effect of hyperlipidaemia on endothelial cell haemostatic properties was examined using ex vivo studies on aortic segments obtained from fat-fed Chinchilla rabbits, mounted in a template device which exposed the luminal surface. Exposure of arterial endothelium to lipids resulted in marked enhancement of externally exposed anionic phospholipids, detected using either fluorescence microscopy with the probe merocyanine 540 or by binding of 125I-polymyxin B and 125I-Annexin V. Consistent with the known procoagulant properties of anionic phospholipid, following the lipid and cholesterol-rich diet intake, intact endothelial cells demonstrated enhanced binding of radioiodinated factors IX/IXa and Xa, and enhanced factor IXa/VIII-dependent factor X activation and factor Xa-factor Va-mediated prothrombin activation. Both factor Xa and thrombin formation were blocked, in large part, by polymyxin B, suggesting dependence of the reaction on anionic phospholipids. Consistent with these results, evidence of increased activation of the coagulation mechanism in vivo was observed in hyperlipidaemic animals, as assessed by a three-fold increase in levels of circulating antithrombin-protease complexes, compared with normolipidaemic controls.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Factores de Coagulación Sanguínea
/
Endotelio Vascular
/
Hipercolesterolemia
Límite:
Animals
Idioma:
En
Revista:
Blood Coagul Fibrinolysis
Asunto de la revista:
ANGIOLOGIA
/
HEMATOLOGIA
Año:
1993
Tipo del documento:
Article
País de afiliación:
Rumanía
Pais de publicación:
Reino Unido