Neutralization of tetanus toxin by human monoclonal antibodies directed against tetanus toxin fragment C.
Hybridoma
; 12(6): 699-708, 1993 Dec.
Article
en En
| MEDLINE
| ID: mdl-8288271
Two hybridomas (designated 143 and 147) producing human monoclonal antibodies (IgG1) directed against tetanus toxin were established by fusion of Epstein-Barr virus transformed human peripheral B lymphocytes with the heteromyeloma SPAM-8. The hybridomas produced antibodies in concentrations of approx. 3.5 micrograms/ml (hybridoma 143) and 6.4 micrograms/ml (hybridoma 147) using conventional flask cultures and 33.9 micrograms/ml and 36.2 micrograms/ml, respectively, in dialysis cultures. The antibodies were shown to react with tetanus toxin, toxoid and fragment C in ELISA, and reactivity with tetanus toxin and fragment C was confirmed in SDS-polyacrylamide gel electrophoresis followed by Western blots. The antibody binding sites were located to two different epitopes of fragment C as shown in a competition assay using biotinylated antibodies. Furthermore, binding of both antibodies to fragment C was inhibited by the addition of the receptor-associated ganglioside GT1b. Neutralization of tetanus toxin in concentrations equivalent to 100-120 IU per mg of antibody was observed for both antibodies in a mouse protection assay.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Toxina Tetánica
/
Inmunoglobulina G
/
Fragmentos Fc de Inmunoglobulinas
/
Anticuerpos Monoclonales
Límite:
Humans
Idioma:
En
Revista:
Hybridoma
Año:
1993
Tipo del documento:
Article
País de afiliación:
Suecia
Pais de publicación:
Estados Unidos