Linkage mapping of 40 randomly isolated liver cDNA clones in the mouse.
Genomics
; 18(2): 295-307, 1993 Nov.
Article
en En
| MEDLINE
| ID: mdl-8288233
We report the chromosomal mapping of 43 loci for 40 randomly isolated mouse liver cDNA clones by linkage analysis in an interspecific backcross of ((C57BL/6J x Mus spretus) x C57BL/6J). The clones were sequenced from both sides and a subset was examined for expression in various mouse tissues. Fifteen of the 40 mapped cDNA clones are either identical or strongly related to known sequences in GenBank, while 25 represent new genes. Additional loci mapped in this cross include 53 simple sequence repeat polymorphisms and 40 restriction fragment length variants from previously characterized cDNA markers. Nine homologous human genes were identified for 7 mouse liver cDNA clones. One clone that maps to mouse chromosome 3 (D3Ucla1) identified a novel homologous segment (synteny) on human chromosome 18q23 (D18S372E). These studies provide linkage mapping and initial characterization of random cDNA clones that may provide a resource for the positional candidate cloning of disease genes.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Mapeo Cromosómico
/
ADN Complementario
/
Hígado
Tipo de estudio:
Clinical_trials
Límite:
Animals
/
Female
/
Humans
Idioma:
En
Revista:
Genomics
Asunto de la revista:
GENETICA
Año:
1993
Tipo del documento:
Article
Pais de publicación:
Estados Unidos