[The immunogenic properties of a recombinant vaccinia virus with an incorporated DNA copy of the 26S RNA of the Venezuelan equine encephalomyelitis virus].

Sviatchenko, V A; Agapov, E V; Urmanov, I Kh; Serpinskii, O I; Frolov, I V; Kolykhalov, A A; Ryzhikov, A B; Netesov, S V

[The immunogenic properties of a recombinant vaccinia virus with an incorporated DNA copy of the 26S RNA of the Venezuelan equine encephalomyelitis virus]. / Immunogennye svoistva rekombinantnogo virusa ospovaktsiny so vstroennoi DNK-kopiei 26S RNK virusa venesuél'skogo éntsefalomielita loshadei.

Sviatchenko, V A; Agapov, E V; Urmanov, I Kh; Serpinskii, O I; Frolov, I V; Kolykhalov, A A; Ryzhikov, A B; Netesov, S V.

Vopr Virusol ; 38(5): 222-6, 1993.

Article en Ru | MEDLINE | ID: mdl-8284922

RESUMEN

A recombinant strain of vaccinia virus (VR26) containing a DNA-copy of the subgenomic 26S RNA of Venezuelan equine encephalomyelitis virus (VEE) inserted into the coding region of thymidine kinase (TK) gene was produced. This subgenomic RNA contained the genes for all structural proteins of the VEE virus, the strain Trinidad donkey (TRD). VR26 effectively expressed VEE virus glycoproteins on the membranes of the infected cells. Blood sera of VR26-immunized animals were found to contain VEE virus-specific antibodies. VR26-immunized mice and rabbits showed a high level of resistance to subcutaneous inoculation with the pathogenic TRD strain of VEE virus. VR26 also provided a high level of protection in animals against aerogenic infection. The absence of virus-neutralizing antibodies in most VR26-immunized animals resistant to inoculation with high doses of VEE suggests the dominant role of the cell component in the immune response. The immune response induced by the recombinant VR26 strain was stable as demonstrated by the resistance of the animals to a challenge with VEE virus 7 months after immunization. The experimental results suggest that this recombinant strain may be considered as a candidate for vaccine preparation.

Asunto(s)

ADN Complementario/genética; ADN Viral/genética; Virus de la Encefalitis Equina Venezolana/genética; Regulación Viral de la Expresión Génica/inmunología; ARN Mensajero/genética; ARN Ribosómico/genética; ARN Viral/genética; Recombinación Genética/inmunología; Virus Vaccinia/inmunología; Animales; Encefalomielitis Equina Venezolana/inmunología; Encefalomielitis Equina Venezolana/prevención & control; Regulación Viral de la Expresión Génica/genética; Sueros Inmunes/inmunología; Inmunización/métodos; Ratones; Conejos; Recombinación Genética/genética; Factores de Tiempo; Vaccinia/inmunología; Vaccinia/prevención & control; Virus Vaccinia/genética

Buscar en Google

Añadir a Mi BVS

Imprimir

XML

PubMed Links

Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Recombinación Genética / Virus Vaccinia / ADN Viral / ARN Mensajero / ARN Ribosómico / ARN Viral / Regulación Viral de la Expresión Génica / ADN Complementario / Virus de la Encefalitis Equina Venezolana Límite: Animals País/Región como asunto: America do sul / Venezuela Idioma: Ru Revista: Vopr Virusol Año: 1993 Tipo del documento: Article Pais de publicación: Rusia

Buscar en Google

Añadir a Mi BVS

Imprimir

XML

PubMed Links