Cloning and characterisation of the cytochrome c gene of Aspergillus nidulans.
Mol Gen Genet
; 242(1): 17-22, 1994 Jan.
Article
en En
| MEDLINE
| ID: mdl-8277943
The cytochrome c gene (cycA) of the filamentous fungus Aspergillus nidulans has been isolated and sequenced. The gene is present in a single copy per haploid genome and encodes a polypeptide of 112 amino acid residues. The nucleotide sequence of the A. nidulans cycA gene shows 87% identity to the DNA sequence of the Neurospora crassa cytochrome c gene, and approximately 72% identity to the sequence of the Saccharomyces cerevisiae iso-1-cytochrome c gene (CYC1). The S. cerevisiae CYC1 gene was used as a heterologous probe to isolate the homologous gene in A. nidulans. The A. nidulans cytochrome c sequence contains two small introns. One of these is highly conserved in terms of position, but the other has not been reported in any of the cytochrome c genes so far sequenced. Expression of the cycA gene is not affected by glucose repression, but has been shown to be induced approximately tenfold in the presence of oxygen and three- to fourfold under heat-shock conditions.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Aspergillus nidulans
/
Proteínas Fúngicas
/
Regulación Fúngica de la Expresión Génica
/
Grupo Citocromo c
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Genes Fúngicos
Idioma:
En
Revista:
Mol Gen Genet
Año:
1994
Tipo del documento:
Article
Pais de publicación:
Alemania