Hormonal study of a human mixed follicular and medullary thyroid carcinoma.
J Mol Endocrinol
; 11(1): 59-67, 1993 Aug.
Article
en En
| MEDLINE
| ID: mdl-8240672
We studied the hormonal secretion of a human mixed follicular and medullary carcinoma. Thyroglobulin (Tg) secretion, especially by large cells and sometimes by small ones, was visualized with immunoenzymatic staining. Calcitonin (CT) was produced by small spindle-shaped cells. Moreover, immunofluorescence double staining performed on the resected thyroid tissue showed the secretion of both Tg and CT in a small number of cells. The cells lost their hormonal secretion after 2 months of culture. Hormonal secretion was modulated by different additives in the medium. Tg secretion was induced when TSH was added to the culture medium; the maximal effect was produced with the addition of 1 mU TSH/ml and 1 microM cortisol, which potentiated the effect of TSH on Tg production. A durable Tg secretion was obtained by embedding the cells in Engelbretch-Hohn-Swarn (EHS) tumour matrix. The CT production was reinduced by the addition of 4 mM Ca2+, 1 microM glucagon and 1 microM cortisol to the culture medium. These findings show that different cells are found in a mixed follicular and medullary carcinoma, some of which can secrete both CT and Tg. They can remain differentiated for a long period after being embedded in EHS tumour matrix with Ca2+ and hormonal components.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Tiroglobulina
/
Calcitonina
/
Neoplasias de la Tiroides
/
Carcinoma Medular
/
Adenocarcinoma Folicular
Límite:
Humans
Idioma:
En
Revista:
J Mol Endocrinol
Asunto de la revista:
BIOLOGIA MOLECULAR
/
ENDOCRINOLOGIA
Año:
1993
Tipo del documento:
Article
País de afiliación:
Francia
Pais de publicación:
Reino Unido