Generation of a mouse strain that produces immunoglobulin kappa chains with human constant regions.
Science
; 262(5137): 1271-4, 1993 Nov 19.
Article
en En
| MEDLINE
| ID: mdl-8235658
Humanized antibodies are highly efficient as immunotherapeutic reagents and have many advantages over rodent antibodies. A mouse strain was generated by gene targeting to replace the mouse kappa light chain constant (C) region gene with the human C kappa gene. Mice homozygous for the replacement mutation (C kappa R) produced normal concentrations of serum antibodies, most of which carry chimeric kappa light chains, and mounted normal immune responses to hapten-protein conjugates. This technology provides a feasible option for the generation of high-affinity humanized antibodies by means of the powerful somatic hypermutation-selection mechanism.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Genes de Inmunoglobulinas
/
Linfocitos B
/
Regiones Constantes de Inmunoglobulina
/
Cadenas kappa de Inmunoglobulina
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Science
Año:
1993
Tipo del documento:
Article
País de afiliación:
Alemania
Pais de publicación:
Estados Unidos