Repetitive sequences in the ribosomal intergenic spacer of Trypanosoma cruzi.

Novak, E M; de Mello, M P; Gomes, H B; Galindo, I; Guevara, P; Ramirez, J L; da Silveira, J F

Novak, E M; de Mello, M P; Gomes, H B; Galindo, I; Guevara, P; Ramirez, J L; da Silveira, J F.

Afiliación

Novak EM; Disciplina de Parasitologia, Escola Paulista de Medicina, São Paulo, Brazil.

Mol Biochem Parasitol ; 60(2): 273-80, 1993 Aug.

Article en En | MEDLINE | ID: mdl-8232418

RESUMEN

A fragment of Trypanosoma cruzi ribosomal intergenic spacer (IGS) located at 6.7 kb from the 3' end of the 24S rRNA gene was analyzed. This IGS fragment is characterized by the presence of three types of repetitive elements (designated Spacer Repetitive Elements, SRE), short direct repeats (5-6 bp) and chi-like recombinational sequences. SRE elements are composed of relatively short repeats (43-145 bp) which show variabilities consisting of nucleotide changes, insertions and deletions. SRE-1 element (145 bp) has a short oligo(dA) tail at the end of the repeat and can be found flanked by other SRE elements. SRE elements are species-specific, suggesting that probes based on them may be diagnostic for Trypanosoma cruzi.

Asunto(s)

Secuencias Repetitivas de Ácidos Nucleicos; Trypanosoma cruzi/genética; Animales; Secuencia de Bases; ADN Protozoario/genética; ADN Ribosómico/genética; Genoma; Datos de Secuencia Molecular; Mapeo Restrictivo; Especificidad de la Especie; Trypanosoma/genética

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Trypanosoma cruzi / Secuencias Repetitivas de Ácidos Nucleicos Límite: Animals Idioma: En Revista: Mol Biochem Parasitol Año: 1993 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Países Bajos

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