Interaction of pyrene-labeled monosialoganglioside GM1 micelles with cholera toxin.

Picking, W D

Picking, W D.

Afiliación

Picking WD; Department of Biology, Saint Louis University, MO 63103-2010.

Biochem Biophys Res Commun ; 195(3): 1153-8, 1993 Sep 30.

Article en En | MEDLINE | ID: mdl-8216243

RESUMEN

Monosialoganglioside GM1 labeled with 1-pyrene-dodecanoic acid was used to monitor interactions between cholera toxin and its receptor. Binding of cholera toxin to labeled ganglioside caused a decrease in pyrene fluorescence intensity at 480 nm concomitant with an increase in fluorescence intensity at 380 and 398 nm. The observed fluorescence changes are similar to those observed when fluorescent ganglioside is moved from an aqueous solvent to an organic solvent. The data are consistent with cholera toxin-bound ganglioside undergoing a process resembling solvent-dependent molecular dispersion.

Asunto(s)

Toxina del Cólera/metabolismo; Gangliósido G(M1)/metabolismo; Receptores de Superficie Celular/metabolismo; Colorantes Fluorescentes/metabolismo; Ácidos Láuricos/metabolismo; Micelas; Movimiento; Espectrometría de Fluorescencia; Factores de Tiempo

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Toxina del Cólera / Receptores de Superficie Celular / Gangliósido G(M1) Idioma: En Revista: Biochem Biophys Res Commun Año: 1993 Tipo del documento: Article Pais de publicación: Estados Unidos

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