The effects of cyclic adenosine monophosphate, forskolin, and theophylline on motility parameters in gossypol-treated human sperm.
Fertil Steril
; 61(5): 929-34, 1994 May.
Article
en En
| MEDLINE
| ID: mdl-8174733
ABSTRACT
PIP: The antimotility effect of gossypol on human sperm has been documented. Gossypol is commonly derived from cottonseed oil. The exact mechanism by which this effect occurs is unknown. This paper reports research on the effects of gossypol on sperm motility. Elucidation of the site(s) of action and whether the effects are reversible are also discussed. Fresh human sperm was collected, centrifuged into pellets of equal numbers, washed, and then either overlayered with Ham's F-10 media or treated with gossypol. Gossypol solutions contained 10, 12.5, 20, 25 or 50 mcg/mcl of gossypol. These concentrations were used in measuring the sperm dose-response. Forskolin, theophylline, or cyclic adenosine monophosphate (cAMP) was added to gossypol-treated sperm and motility levels were measured. Motility measurements were conducted manually or via computer-assisted readings of sperm motion characteristics and their actual ability to move. Antimotility effects of gossypol on sperm are related to both dose and exposure time. This study supports the hypothesis that gossypol inhibits cAMP formation. At lower doses of gossypol (10 mcg/mcl), both theophylline and forskolin reversed gossypol's antimotility effect. At higher concentrations (20 mcg/mcl), the effect of gossypol appeared to be rapid and was irreversible. This latter finding has implications for its use as a vaginal contraceptive agent.
Palabras clave
Americas; Biology; Clinical Research; Contraception; Contraception Research; Contraceptive Agents; Contraceptive Agents, Male; Contraceptive Mode Of Action; Developed Countries; Family Planning; Genitalia; Germ Cells; Gossypol; In Vitro; Inhibition Of Fertilization; Kentucky; North America; Northern America; Physiology; Research Methodology; Research Report; Sperm Transport Inhibition; Spermatozoa; United States; Urogenital System
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Motilidad Espermática
/
Espermatozoides
/
Teofilina
/
Colforsina
/
Gosipol
/
AMP Cíclico
Límite:
Humans
/
Male
Idioma:
En
Revista:
Fertil Steril
Año:
1994
Tipo del documento:
Article
Pais de publicación:
Estados Unidos