Identification of a genomic fragment of Mycobacterium tuberculosis responsible for in vivo growth advantage.
Infect Agents Dis
; 2(4): 282-4, 1993 Aug.
Article
en En
| MEDLINE
| ID: mdl-8173811
The purpose of this work was to develop a system to identify virulence determinants of M. tuberculosis by genetic complementation. The ability to grow in mouse spleen and/or lung was defined as a potential phenotype for virulence. Enrichment for growing recombinant clones from a pool of H37Ra transformants containing the integrating pYUB178::H37Rv cosmid library was accomplished by in vivo selection. A molecular strategy was devised to isolate and clone the 25-kb H37Rv genomic fragment ivg that conferred in vivo growth advantage to H37Ra. This study is a first step toward understanding the genetics of virulence in M. tuberculosis. A detailed description of these experiments has been submitted for publication in Infection and Immunity.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Genes Bacterianos
/
Mycobacterium tuberculosis
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
En
Revista:
Infect Agents Dis
Asunto de la revista:
DOENCAS TRANSMISSIVEIS
Año:
1993
Tipo del documento:
Article
Pais de publicación:
Estados Unidos