Release of potassium, lipids, and proteins from nonionic detergent treated chicken red blood cells.
J Cell Physiol
; 159(2): 197-204, 1994 May.
Article
en En
| MEDLINE
| ID: mdl-8163560
The plasma membrane of erythrocytes, as of other cells, is thought to act as the barrier responsible for maintaining intracellular gradients of most ions and small molecular species between the cell and its environment. Controlled application of the nonionic detergent Brij 58 effectively opened the erythrocyte plasma membrane, as judged by electron microscopy and lipid mobilization, but the cytoplasm maintained much of its integrity for about 30 min. Release of K+ correlated well with release of protein into the surrounding medium. The results demonstrate that permeabilization of the erythrocyte plasma membrane does not result in an instantaneous equilibration of small ions, such as K+, between the cell and its environment. A comparison was made between erythrocytes treated with Brij 58 and Triton X-100. The lipid and protein solubilizing actions of Triton X-100 were not as easily separable in time as those of Brij 58. The results of treatment of the erythrocytes with different types of nonionic detergents suggest that the membranolytic and cytoplasmic protein destabilizing actions of nonionic detergents correspond with their hydrophilic-lipophilic balance numbers (HLB values).
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Potasio
/
Proteínas Sanguíneas
/
Octoxinol
/
Eritrocitos
/
Lípidos
Límite:
Animals
Idioma:
En
Revista:
J Cell Physiol
Año:
1994
Tipo del documento:
Article
País de afiliación:
Hungria
Pais de publicación:
Estados Unidos