Two intestinal specific nuclear factors binding to the lactase-phlorizin hydrolase and sucrase-isomaltase promoters are functionally related oligomeric molecules.
FEBS Lett
; 342(3): 297-301, 1994 Apr 11.
Article
en En
| MEDLINE
| ID: mdl-8150088
Lactase-phlorizin hydrolase (LPH) and sucrase-isomaltase (SI) are enterocyte-specific gene products. The identification of regulatory cis-elements in the promoter of these two genes has enabled us to carry out comparative studies of the corresponding intestinal-specific nuclear factors (NF-LPH1 and SIF1-BP). Electrophoretic mobility shift assays demonstrated that the two nuclear factors compete for binding on the same cis-elements. The molecular size of the DNA binding polypeptide is estimated to be approximately 50 kDa for both factors. In the native form the factors are found as 250 kDa oligomeric complexes. Based on these results NF-LPH1 and SIF1-BP are suggested to be either identical or closely related molecules.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Complejo Sacarasa-Isomaltasa
/
Proteínas Nucleares
/
Lactasa-Florizina Hidrolasa
/
Proteínas de Unión al ADN
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
FEBS Lett
Año:
1994
Tipo del documento:
Article
País de afiliación:
Dinamarca
Pais de publicación:
Reino Unido