1 kb of the lactase-phlorizin hydrolase promoter directs post-weaning decline and small intestinal-specific expression in transgenic mice.

Troelsen, J T; Mehlum, A; Olsen, J; Spodsberg, N; Hansen, G H; Prydz, H; Norén, O; Sjöström, H

Troelsen, J T; Mehlum, A; Olsen, J; Spodsberg, N; Hansen, G H; Prydz, H; Norén, O; Sjöström, H.

Afiliación

Troelsen JT; Department of Medical Biochemistry and Genetics, Panum Institute, University of Copenhagen, Denmark.

FEBS Lett ; 342(3): 291-6, 1994 Apr 11.

Article en En | MEDLINE | ID: mdl-8150087

RESUMEN

Adult-type hypolactasia is a genetic condition making approximately one half of the human population intolerant to milk because of abdominal symptoms. The cause is a post-weaning down-regulation of the intestinal-specific enzyme lactase-phlorizin hydrolase (LPH) reducing the intestinal capacity to hydrolyze lactose. We here demonstrate that the stretch -17 to -994 in the pig LPH-promoter carries cis-elements which direct a small intestinal-specific expression and a post-weaning decline of a linked rabbit beta-globin gene. These data demonstrate that the post-weaning decline of LPH is mainly due to a transcriptional down-regulation.

Asunto(s)

Regulación de la Expresión Génica; Intestino Delgado/enzimología; Lactasa-Florizina Hidrolasa/genética; Factores de Edad; Animales; Ratones; Ratones Endogámicos C57BL; Ratones Endogámicos DBA; Ratones Transgénicos; Regiones Promotoras Genéticas; ARN Mensajero/genética; Distribución Tisular; Transcripción Genética

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Regulación de la Expresión Génica / Lactasa-Florizina Hidrolasa / Intestino Delgado Límite: Animals Idioma: En Revista: FEBS Lett Año: 1994 Tipo del documento: Article País de afiliación: Dinamarca Pais de publicación: Reino Unido

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