Rescue by vitamin B12 of Escherichia coli cells treated with colicins A and E allows measurement of the kinetics of colicin binding on BtuB.
FEMS Microbiol Lett
; 116(1): 37-42, 1994 Feb 01.
Article
en En
| MEDLINE
| ID: mdl-8132153
Sensitivity of Escherichia coli bacteria to colicins A and E1 was significantly increased by overproduction of the BtuB receptor protein. The amount of vitamin B12 needed before colicins A and E1 treatment to protect cells against killing was found to be a function of the number of BtuB molecules present at the cell surface. Cells treated by colicins A and E were rescued from killing by addition of vitamin B12 shortly after colicin treatment. The rate of reversal by vitamin B12 may correspond to the kinetics of irreversible binding to BtuB of the various colicins.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas de la Membrana Bacteriana Externa
/
Vitamina B 12
/
Receptores de Péptidos
/
Colicinas
/
Proteínas de Escherichia coli
/
Escherichia coli
Idioma:
En
Revista:
FEMS Microbiol Lett
Año:
1994
Tipo del documento:
Article
País de afiliación:
Francia
Pais de publicación:
Reino Unido