High-resolution proton nuclear magnetic resonance spectroscopy in the detection of low molecular weight volatiles.
J Anal Toxicol
; 17(5): 273-7, 1993 Sep.
Article
en En
| MEDLINE
| ID: mdl-8107460
Proton nuclear magnetic resonance spectroscopy (1H MRS) has been used to identify ethanol in vivo and to detect other exogenous low molecular weight volatiles in human serum. 1H MRS was used to detect and quantitate 15 human sera containing various concentrations and combinations of ethanol, isopropanol, acetone, and methanol as previously quantitated by headspace gas chromatography. The 1H MRS method was linear for each alcohol. The lowest detectable alcohol concentration was 15 mg/L (peak height equal to three times the signal-to-noise ratio), and 30 mg/L (+/- 10% relative standard deviation) was the lowest level reproducibly quantitated. Within-run and day-to-day coefficients of variation (CV) ranged from 0.8 to 2.0% and 0.9 to 1.2%, respectively, for methanol; 0.5 to 1.9% and 0.6 to 1.3% for acetone; and 0.5 to 1.6% and 0.3 to 2.2% for isopropanol. In all cases, the lowest CVs for a particular compound were obtained for the highest measured concentration (1500 mg/L), and the highest CVs were observed for the lowest concentration (250 mg/L). The 1H MRS method for detection of these volatiles does not require sample pretreatment and is nondestructive, which allows for further analysis by other methods.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Acetona
/
Espectroscopía de Resonancia Magnética
/
Etanol
/
Metanol
/
1-Propanol
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Revista:
J Anal Toxicol
Año:
1993
Tipo del documento:
Article
Pais de publicación:
Reino Unido