Use of a 35.5 kDa cell membrane composition of Pasteurella multocida and an anti-idiotype antibody to induce protective immunity in leghorn chickens.
Vet Immunol Immunopathol
; 41(1-2): 89-100, 1994 May.
Article
en En
| MEDLINE
| ID: mdl-8066998
Using polyacrylamide gel electrophoresis and Western blot analysis, a 35.5 kDa cell membrane composition of Pasteurella multocida ATCC 11039 was identified as a dominant epitope of the bacterium. Mice inoculated with inactivated whole bacteria produce antisera primary reactive with the 35.5 kDa component (Pm35.5) in Western blot analysis. The outer membrane component was composed primarily of protein but did have lipopolysaccharide present at 133 micrograms mg-1 protein. In challenge trials (Trial 2), groups of white leghorn chickens vaccinated with Pm35.5 or an anti-idiotypic antibody (using Pm35.5 as the original antigen) had significant difference in mortality of 3.2% and 48.3%, respectively, when compared with unvaccinated controls (99%). Mortality in a group of chickens receiving a commercially available bacterin (9.2%) was higher but not significantly different from the mortality of the Pm35.5 vaccinated group (3.2%).
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Infecciones por Pasteurella
/
Enfermedades de las Aves de Corral
/
Proteínas de la Membrana Bacteriana Externa
/
Pollos
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Anticuerpos Antiidiotipos
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Vacunación
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Pasteurella multocida
Límite:
Animals
Idioma:
En
Revista:
Vet Immunol Immunopathol
Año:
1994
Tipo del documento:
Article
Pais de publicación:
Países Bajos