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Carbohydrate dynamics at a micellar surface: GD1a headgroup transformations revealed by NMR spectroscopy.
Poppe, L; van Halbeek, H; Acquotti, D; Sonnino, S.
Afiliación
  • Poppe L; Complex Carbohydrate Research Center, University of Georgia, Athens 30602.
Biophys J ; 66(5): 1642-52, 1994 May.
Article en En | MEDLINE | ID: mdl-8061213
The conformational dynamics of the carbohydrate headgroup of ganglioside GD1a, NeuAc alpha 2-->3Gal beta 1-->3GalNAc beta 1-->4[NeuAc alpha 2-->3]Gal beta 1-->4Glc beta 1-->1Cer, anchored in a perdeuterated dodecylphosphocholine micelle in aqueous solution, were probed by high resolution NMR spectroscopy. The observed 1H/1H NOE interactions revealed conformational averaging of the terminal NeuAc alpha 2-->3Gal and Gal beta 1-->3GalNAc glycosidic linkages. The pronounced flexibility of this trisaccharide moiety was substantiated further by two-dimensional proton-detected 13C T1, T1 rho and 1H/13C NOE measurements. The anchoring effect of the micelle allowed the detection of conformational fluctuations of the headgroup on the time scale of a few hundred picoseconds. NMR experiments performed on the GD1a/DPC micelles in H2O at low temperatures permitted the observation of hydroxyl proton resonances, contributing valuable conformational information.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Gangliósidos Límite: Animals Idioma: En Revista: Biophys J Año: 1994 Tipo del documento: Article Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Gangliósidos Límite: Animals Idioma: En Revista: Biophys J Año: 1994 Tipo del documento: Article Pais de publicación: Estados Unidos