Secretion of bioactive granulocyte-macrophage colony-stimulating factor by human colorectal carcinoma cells.

Lahm, H; Wyniger, J; Hertig, S; Yilmaz, A; Fischer, J R; Givel, J C; Odartchenko, N

Lahm, H; Wyniger, J; Hertig, S; Yilmaz, A; Fischer, J R; Givel, J C; Odartchenko, N.

Afiliación

Lahm H; Department of Cellular Biology, Swiss Institute for Experimental Cancer Research, Epalinges.

Cancer Res ; 54(14): 3700-2, 1994 Jul 15.

Article en En | MEDLINE | ID: mdl-8033086

RESUMEN

Secretion of several cytokines by colorectal carcinoma cells has been substantiated. These do not include granulocyte-macrophage colony-stimulating factor (GM-CSF) thus far. We show that the supernatant of two human colorectal carcinoma cell lines, LS1034 and SW480, stimulates proliferation of GM-CSF-dependent M07e cells. The activity was constitutively secreted by LS1034 cells and could be induced by serum-free culture conditions in SW480 cells. Addition of a neutralizing anti-GM-CSF antibody completely inhibited this activity. Preabsorption with anti-GM-CSF antibody removed all M07e growth-stimulating activity from LS1034 and SW480 supernatant. Western blot analysis revealed the presence of GM-CSF in LS1034 supernatant. Our results indicate that human colorectal carcinoma cells secrete indeed biologically active GM-CSF.

Asunto(s)

Neoplasias Colorrectales/metabolismo; Factor Estimulante de Colonias de Granulocitos y Macrófagos/metabolismo; Neoplasias Colorrectales/patología; Humanos; Células Tumorales Cultivadas

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Colorrectales / Factor Estimulante de Colonias de Granulocitos y Macrófagos Límite: Humans Idioma: En Revista: Cancer Res Año: 1994 Tipo del documento: Article Pais de publicación: Estados Unidos

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