Phosphate regulation of biosynthesis of extracellular RNases of endospore-forming bacteria.

Znamenskaya, L V; Gabdrakhmanova, L A; Chernokalskaya, E B; Leshchinskaya, I B; Hartley, R W

Znamenskaya, L V; Gabdrakhmanova, L A; Chernokalskaya, E B; Leshchinskaya, I B; Hartley, R W.

Afiliación

Znamenskaya LV; Department of Microbiology, Kazan State University, Russian Federation.

FEBS Lett ; 357(1): 16-8, 1995 Jan 02.

Article en En | MEDLINE | ID: mdl-8001670

RESUMEN

The gene for the extracellular ribonuclease of B. pumilus KMM62 (RNase Bp) has been cloned and sequenced. The structural gene for this enzyme is similar to those of the extracellular ribonucleases of B. intermedius 7P (binase) and B. amyloliquefaciens H2 (barnase), as are the regulatory regions of binase and RNase Bp. The regulatory region of the barnase gene, however, is quite different from the other two. In the promoter of the genes for binase and RNase Bp, but not in that for barnase, is a region similar to the Pho box of E. coli. We have established that inorganic phosphate suppresses the synthesis of the binase and RNase Bp, but does not effect the synthesis of barnase.

Asunto(s)

Bacillus/enzimología; Fosfatos/metabolismo; Ribonucleasas/biosíntesis; Secuencia de Aminoácidos; Bacillus/genética; Bacillus/fisiología; Proteínas Bacterianas; Secuencia de Bases; Clonación Molecular; ADN Bacteriano; Endorribonucleasas/genética; Genes Bacterianos; Datos de Secuencia Molecular; Ribonucleasas/genética; Esporas Bacterianas

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fosfatos / Ribonucleasas / Bacillus Idioma: En Revista: FEBS Lett Año: 1995 Tipo del documento: Article Pais de publicación: Reino Unido

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