Highly-efficient transformation of the homobasidiomycete Schizophyllum commune to phleomycin resistance.
Curr Genet
; 26(2): 179-83, 1994 Aug.
Article
en En
| MEDLINE
| ID: mdl-8001174
Regulatory sequences of the glyceraldehyde-3-phosphate-dehydrogenase (GPD) gene from the homobasidiomycete Schizophyllum commune were fused to the coding sequence of the ble gene from Streptoalloteichus hindustanus, which codes for a phleomycin-binding protein. The resulting construct transformed S. commune to phleomycin resistance at a high frequency (up to 10(4) transformants/microgram DNA per 10(7) protoplasts) when regeneration was done in 0.5 M MgSO4. A similar construct with regulatory sequences from Aspergillus nidulans failed to give transformants, showing the importance of homologous regulatory sequences for the expression of genes in S. commune. The homologous GPD promoter could be deleted up to position -130 without any effect on the number of phleomycin-resistant transformants. This is the first effective stable transformation system in a homobasidiomycete employing antibiotic resistance.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Schizophyllum
/
Transformación Genética
/
Farmacorresistencia Microbiana
/
Gliceraldehído-3-Fosfato Deshidrogenasas
Idioma:
En
Revista:
Curr Genet
Año:
1994
Tipo del documento:
Article
País de afiliación:
Países Bajos
Pais de publicación:
Estados Unidos