Metallothionein-II and ferritin H mRNA levels are increased in arsenite-exposed HeLa cells.

Guzzo, A; Karatzios, C; Diorio, C; DuBow, M S

Guzzo, A; Karatzios, C; Diorio, C; DuBow, M S.

Afiliación

Guzzo A; McGill University, Department of Microbiology and Immunology, Montreal, Quebec, Canada.

Biochem Biophys Res Commun ; 205(1): 590-5, 1994 Nov 30.

Article en En | MEDLINE | ID: mdl-7999084

RESUMEN

Arsenite is extremely toxic and, though non-mutagenic, is a carcinogen. To determine the effects of arsenite on changes in cell physiology, we searched for genes in HeLa cells whose mRNAs are more abundant after cellular exposure to arsenite. A cDNA subtraction was performed between cDNA synthesized from HeLa cells grown in the absence and presence of 5 microM sodium arsenite. Isolation and sequencing of three clones that showed a higher hybridization signal to RNA from arsenite-exposed cells, versus unexposed cells, revealed that two of the cDNAs coded for human ferritin H chain and the other coded for metallothionein-II. These results suggest the possibility that arsenite exposure may lead to increased levels of oxygen radicals, which augmented metallothionein and ferritin can act to detoxify.

Asunto(s)

Arsenitos/toxicidad; Ferritinas/genética; Metalotioneína/genética; ARN Mensajero/metabolismo; ADN Complementario; Expresión Génica/efectos de los fármacos; Células HeLa; Humanos

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ARN Mensajero / Arsenitos / Ferritinas / Metalotioneína Límite: Humans Idioma: En Revista: Biochem Biophys Res Commun Año: 1994 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Estados Unidos

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