Sites in human nuclei where damage induced by ultraviolet light is repaired: localization relative to transcription sites and concentrations of proliferating cell nuclear antigen and the tumour suppressor protein, p53.
J Cell Sci
; 107 ( Pt 7): 1753-60, 1994 Jul.
Article
en En
| MEDLINE
| ID: mdl-7983145
The repair of damage induced in DNA by ultraviolet light involves excision of the damaged sequence and synthesis of new DNA to repair the gap. Sites of such repair synthesis were visualized by incubating permeabilized HeLa or MRC-5 cells with the DNA precursor, biotin-dUTP, in a physiological buffer; then incorporated biotin was immunolabeled with fluorescent antibodies. Repair did not take place at sites that reflected the DNA distribution; rather, sites were focally concentrated in a complex pattern. This pattern changed with time; initially intense repair took place at transcriptionally active sites but when transcription became inhibited it continued at sites with little transcription. Repair synthesis in vitro also occurred in the absence of transcription. Repair sites generally contained a high concentration of proliferating cell nuclear antigen but not the tumour-suppressor protein, p53.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Transcripción Genética
/
Rayos Ultravioleta
/
Daño del ADN
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ADN
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Núcleo Celular
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Proteína p53 Supresora de Tumor
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Antígeno Nuclear de Célula en Proliferación
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Reparación del ADN
Límite:
Humans
Idioma:
En
Revista:
J Cell Sci
Año:
1994
Tipo del documento:
Article
Pais de publicación:
Reino Unido