HSP 90 alpha and HSP 90 beta genes are present in the zebrafish and are differentially regulated in developing embryos.
Biochem Biophys Res Commun
; 204(2): 746-52, 1994 Oct 28.
Article
en En
| MEDLINE
| ID: mdl-7980538
We have employed a polymerase chain reaction-based cloning strategy to demonstrate that both hsp 90 alpha and hsp 90 beta genes are present in the zebrafish. The fact that zebrafish represents the most primitive vertebrate in which hsp 90 genes have been isolated to date has allowed us to determine that the duplication event which generated the hsp 90 alpha and hsp 90 beta genes occurred shortly before the emergence of the teleosts from the rest of the vertebrate lineage. In expression studies using Northern blot analysis, hsp 90 beta mRNA was found to be present at control temperatures throughout normal embryonic development whereas hsp 90 alpha mRNA was barely detectable. Upon heat shock, hsp 90 alpha mRNA levels increased dramatically in all developmental stages examined. The levels of hsp 90 beta mRNA increased 2-3 fold during heat shock of early stage embryos. Thus, the hsp 90 alpha gene is strongly upregulated during heat shock in zebrafish embryos whereas expression of the hsp 90 beta gene appears to be weakly induced.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Pez Cebra
/
Proteínas HSP90 de Choque Térmico
/
Regulación del Desarrollo de la Expresión Génica
Límite:
Animals
Idioma:
En
Revista:
Biochem Biophys Res Commun
Año:
1994
Tipo del documento:
Article
País de afiliación:
Canadá
Pais de publicación:
Estados Unidos