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Nucleolar size polymorphisms in commercial layer chickens; determination of incidence, inheritance, and nucleolar sizes.
Delany, M E; Emsley, A; Smiley, M B; Putnam, J R; Bloom, S E.
Afiliación
  • Delany ME; Department of Avian and Aquatic Animal Medicine, Cornell University, Ithaca, New York 14853.
Poult Sci ; 73(8): 1211-7, 1994 Aug.
Article en En | MEDLINE | ID: mdl-7971662
In the chicken, one chromosome pair encodes the ribosomal (r)RNA genes and two nucleoli are formed in interphase cells. The nucleolus is the site of rRNA synthesis and ribosome biogenesis. Nucleolar size polymorphisms have been detected in research strains of chickens and found to represent heterozygosity for rRNA gene copy number. The objectives of the present study were to determine whether nucleolar size polymorphisms exist in commercial chicken flocks and whether such polymorphisms are under genetic control. The occurrence of nucleolar size polymorphisms was studied in three lines of commercial layer chickens (designated A, B, and C). Nucleolar size polymorphisms were found in all three lines. However, the lines differed in the proportion of individuals exhibiting the polymorphic phenotype of two unequal-sized nucleoli (2P). The 2P phenotype, determined in successive years, was found in 10 to 14% of Line A birds, 18 to 23% of Line B birds, and 41 to 63% of Line C birds. The inheritance pattern of the nucleolar size polymorphism and nucleoli sizes were studied in Line C birds. The 2P phenotype was found to be inherited in Mendelian fashion. The large nucleolus was 1.7x and 1.6x that of the smaller nucleolus in 2P males and females, respectively, and was larger than the nucleoli of 2E birds (2E = nonpolymorphic phenotype of two equal-sized nucleoli). Total nucleolar size was greater in cells of 2P birds than in cells of 2E birds. These data suggest that the genetic basis for the large nucleolus in Line C 2P cells is a "larger than normal" rDNA cluster.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polimorfismo Genético / Nucléolo Celular / Pollos Tipo de estudio: Incidence_studies / Prognostic_studies / Risk_factors_studies Límite: Animals Idioma: En Revista: Poult Sci Año: 1994 Tipo del documento: Article Pais de publicación: Reino Unido
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polimorfismo Genético / Nucléolo Celular / Pollos Tipo de estudio: Incidence_studies / Prognostic_studies / Risk_factors_studies Límite: Animals Idioma: En Revista: Poult Sci Año: 1994 Tipo del documento: Article Pais de publicación: Reino Unido