Surfactant protein A and saturated phosphatidylcholine in respiratory distress syndrome.

Moya, F R; Montes, H F; Thomas, V L; Mouzinho, A M; Smith, J F; Rosenfeld, C R

Moya, F R; Montes, H F; Thomas, V L; Mouzinho, A M; Smith, J F; Rosenfeld, C R.

Afiliación

Moya FR; Department of Pediatrics, University of Texas Southwestern Medical Center at Dallas 75235.

Am J Respir Crit Care Med ; 150(6 Pt 1): 1672-7, 1994 Dec.

Article en En | MEDLINE | ID: mdl-7952631

RESUMEN

We measured surfactant protein A (SP-A) by ELISA using a rabbit antihuman SP-A polyclonal antibody and saturated phosphatidylcholine (SPC) by thin-layer chromatography in sequential tracheal fluid samples obtained from 16 preterm neonates without lung disease and 37 with respiratory distress syndrome (RDS). SP-A and SPC were lower in neonates with RDS than in control infants (1.0 +/- 0.1 versus 8.9 +/- 2.2 ng SP-A/microgram protein [p < 0.0001] and 0.20 +/- 0.05 versus 0.70 +/- 0.19 mumol SPC/mg protein [p < 0.01], respectively). Initial SP-A concentrations correlated inversely with severity of RDS (r = 0.45, p < 0.01) but did not correlate with initial SPC levels. Significant increases in SP-A were detectable within 12 to 24 h after birth in neonates with RDS. Further increases occurred subsequently and were similar for neonates treated with either a synthetic (Exosurf) or a modified natural (Survanta) surfactant. Using two-dimensional gel electrophoresis, SP-A in tracheal fluid obtained during the early and recovery phases of RDS exhibited lesser degrees of posttranslational modification than SP-A forms from control neonates. Administration of Exosurf or Survanta resulted in comparable increases in SPC in tracheal fluid. Preterm neonates with RDS seem to have an immature SP-A metabolism that persists for several days after birth. The type of surfactant used does not modify the recovery of SP-A or SPC in tracheal fluid from infants with RDS.

Asunto(s)

Glicoproteínas/metabolismo; Fosfatidilcolinas/metabolismo; Proteolípidos/metabolismo; Surfactantes Pulmonares/metabolismo; Síndrome de Dificultad Respiratoria del Recién Nacido/metabolismo; Análisis de Varianza; Femenino; Glicoproteínas/análisis; Glicoproteínas/efectos de los fármacos; Humanos; Recién Nacido; Recien Nacido Prematuro; Masculino; Fosfatidilcolinas/análisis; Proteolípidos/análisis; Proteolípidos/efectos de los fármacos; Proteína A Asociada a Surfactante Pulmonar; Proteínas Asociadas a Surfactante Pulmonar; Surfactantes Pulmonares/administración & dosificación; Surfactantes Pulmonares/análisis; Surfactantes Pulmonares/efectos de los fármacos; Síndrome de Dificultad Respiratoria del Recién Nacido/tratamiento farmacológico; Estadísticas no Paramétricas; Factores de Tiempo; Tráquea/metabolismo

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fosfatidilcolinas / Proteolípidos / Síndrome de Dificultad Respiratoria del Recién Nacido / Surfactantes Pulmonares / Glicoproteínas Límite: Female / Humans / Male / Newborn Idioma: En Revista: Am J Respir Crit Care Med Asunto de la revista: TERAPIA INTENSIVA Año: 1994 Tipo del documento: Article Pais de publicación: Estados Unidos

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