A refined guinea pig model for evaluating delayed-type hypersensitivity reactions caused by Q fever vaccines.
Lab Anim Sci
; 44(6): 608-12, 1994 Dec.
Article
en En
| MEDLINE
| ID: mdl-7898035
The previously established model for determining dermal granulomatous reactivity of Q fever vaccines uses footpad injections to sensitize Hartley guinea pigs. We describe a substantial refinement to that model by substituting an alternate injection route and replacing the model with the hairless Hartley guinea pig. The experimental design incorporates a matrix of various antigen and adjuvant combinations administered by subcutaneous (SC) and intradermal routes of administration. The two Coxiella burnetii antigens evaluated were phase-I whole-cell vaccine (WCV) and phase-I chloroform-methanol residue vaccine. The adjuvants used were complete Freund's (CFA), incomplete Freund's, and RIBI's De-Tox. Phase-I C. burnetii WCV in CFA administered SC provided the most efficacious regimen evaluated for sensitizing hairless guinea pigs. The establishment of this animal model allows evaluation of candidate Q fever vaccines in a more humane model than that previously available.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Fiebre Q
/
Vacunas Bacterianas
/
Coxiella burnetii
/
Modelos Animales de Enfermedad
/
Hipersensibilidad Tardía
Límite:
Animals
Idioma:
En
Revista:
Lab Anim Sci
Año:
1994
Tipo del documento:
Article
Pais de publicación:
Estados Unidos