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Astrocytes from adult rat optic nerves are nonpermissive for regenerating retinal ganglion cell axons.
Bähr, M; Przyrembel, C; Bastmeyer, M.
Afiliación
  • Bähr M; Neurologische Universitätsklinik, Tübingen, Federal Republic of Germany.
Exp Neurol ; 131(2): 211-20, 1995 Feb.
Article en En | MEDLINE | ID: mdl-7895822
We have directly compared the abilities of astrocytes from newborn and adult rats to support or inhibit the growth of regenerating axons in vitro. Astrocytes prepared from newborn rats were able to promote retinal ganglion cell (RGC) axon growth from embryonic and adult rat and from adult fish retinal explants. Retinal axons from E16 rat retinae grew significantly faster on astrocytes from neonatal rats than those from E18 or adult rat retinae with growth rates comparable to RGC axons from adult fish retinae. RGC regeneration from adult rat retinae was almost completely inhibited on adult rat optic nerve astrocytes. Only axons from adult fish retinae were able to extend onto monolayers from these reactive astrocytes, although their growth rates were significantly reduced. We conclude that the failure of mammalian RGC axons to regrow within the lesioned optic nerve environment is, at least in part, due to nonpermissive aspects of adult "reactive" optic nerve astrocytes. However, the cell intrinsic growth potential of RGCs also appears to influence their ability to extend axons on cellular substrates.
Asunto(s)
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Nervio Óptico / Células Ganglionares de la Retina / Axones / Astrocitos / Traumatismos del Nervio Óptico / Regeneración Nerviosa Límite: Animals Idioma: En Revista: Exp Neurol Año: 1995 Tipo del documento: Article Pais de publicación: Estados Unidos
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Nervio Óptico / Células Ganglionares de la Retina / Axones / Astrocitos / Traumatismos del Nervio Óptico / Regeneración Nerviosa Límite: Animals Idioma: En Revista: Exp Neurol Año: 1995 Tipo del documento: Article Pais de publicación: Estados Unidos